Hematology and Oncology

Biography

Biography: Artem Dakhovnyk

Abstract

Multiple sclerosis (MS) is an autoimmune disorder of the central nervous system. A lot of findings support the prominent role of the coagulation system in development of MS. It is well known the IgG levels are higher in patients with MS.

This study aimed to determine whether effect of IgG, accumulated in the bloodstream after suffering the MS, on the amidolytic activity of anticoagulation factor. Further to compare their effect with the analogical effect of healthy donor's IgG.

IgG was separated by affinity chromatography on protein A Sepharose from the blood plasma samples obtained from 35 healthy donors and 20 patients with multiple sclerosis. All donors and patients or their relatives had been informed consent in accordance with the Declaration of Helsinki and a protocol approved by the Ethics Committees of the ESC “Institute of Biology and Medicine”, Kyiv, Ukraine.

To examine the IgG influence on PC during its zymogens activation the mixture was prepared: 25μl of healthy donor's plasma was mixed with vehicle, and 25 μl of activators derived from the venom Agkistrodon blomhoffi ussuriensis and then IgG (in a concentration 100 or 300 mkg/ml) was added. After 5 min incubation at 37° C chromogenic substrate S₂₃₆₆ in final concentration 0,3 mM was added. Registration of absorption was carried out in two-wave mode at the primary 405 and references 492 nm wavelength by the spectrophotometer during 60 minutes. Control sample included vehicle instead of IgG.

Results showed the ability of IgG to affect the amidolytic activity of hemostasis enzymes such protein C activated from zymogens in plasma. The statistically significant was observed under the influence of MS IgG in a concentration 300 mkg/ml. Thus the level of PC activation was elevated of the 21 % comparing to the control. 

The observed impact of MS IgG on the PC may therefore provide future targets for therapeutic strategies.