3^rd International Conference on Hematology & Blood Disorders November 02-04, 2015 Atlanta, USA

Currently working in academics with interest in research & hematology.

Sickle-cell disease (SCD) is an autosomal recessive genetic blood disorder. Coenzyme Q10 is an oil-soluble, vitamin-like substance. The aim was to explore the possible role of coenzyme Q10 in improving the treatment and prognosis in sickle cell patient community. The first step towards this goal was to determine the levels of Coenzyme Q10 in sickle cell subjects. In this case controlled study of sickle cell disease patients and healthy matched controls, male and females were in 1:1 ratio. Both groups were submitted to data collection regarding age, sex, height, weight and average number of hospitalisations. Various haematological and physiological parameters were measured. Quantitative estimations of Coenzyme Q10 levels were done for both groups. Levels of Coenzyme Q10 were remarkably lower in sickle cell subjects as compared to normal ones. The levels of CoQ10 reported in this study are in the range of 1160 ngm/ml to 2309 ngm/ml for normal subjects while that among sickle cell subjects lies within a range of 650-1264 ngm/ml. On comparing the levels of CoQ10 with age, it was clearly evident in both males and females within each age subgroup that after initial increase, the levels of CoQ10 decreases with age. It can be concluded that Coenzyme Q10 levels showed significant variation between normal and sickle cell subjects and owing to its role in cellular bioenergetics and free radical metabolism, further research is needed to explore the effect of CoQ10 in sickle cell patients.

Hulya Cabadak is an Associate Professor at Marmara University, Turkey. Her research areas are Cancer Research, Cell Biology, Biology

Acetylcholine is a neurotransmitter in the nervous system but it serves also as a paracrine or autocrine factor in different cell types, where it is linked to functions like proliferation and cell differentiation. Muscarinic receptors are relatively abundant in the central nervous system and peripheral parasympathetic nervous system. Many cells express a mixture of muscarinic receptor transcripts. Changes in muscarinic M2 and M3 receptor mRNA levels in response to agonist treatment have been reported in cerebellar granule cells, Chinese hamster ovary cells, lymphocytes and in the human neuroblasto macellline SH-SY5Y. Several researchers have suggested that non-neuronalacetylcholine and cholinergic agonists alter cell growth and proliferation of lymphocytes. Costa et al. demonstrated that acetylcholine released from T-lymphocytes acts via the M3 acetylcholine muscarinic receptor (mAChR) to trigger nuclear signaling and up-regulation of gene expression in T and B-lymphocytes. We have previously demonstrated the presence of M2, M3 and M4 mAChRs and M3 subtype mediated NO signaling in K562 chronic myelogenous leukemic cells. We also showed that carbacho (CCh), cholinergic agonist, treatment leads to changes in muscarinic M2, M3 and M4 receptor transcripts as well as M2 and M3 protein levels and enhances cyclic adenosine monophosphate (cAMP) accumulation in K562 cells. In this study, we investigated the levels of muscarinic receptor protein expression in megacaryocytic differentiated K562 leukemia cells. And we also investigated the effects of agonist stimulation on megacaryocytic differentiated K562 leukemia cells proliferation. Muscarinic cholinergic receptors activate stimulatory growth mechanisms in megacaryocytic differentiated K562 leukemia cells. stimulation on cell proliferation and expression in megacaryocytic differentiated K562 leukemia cells. We also found that CCh changed proliferation of megacaryocytic differentiated K562 cells proliferation in 24 hour, These results suggest that CCh modulates megacaryocytic differentiated K562 leukemic cells proliferation through muscarinic acetylcholine receptorsrnWe showed that CCh-treatment leads to changes in muscarinic M2, M3, and M4 receptor transcripts as well as M2 and M3 protein levels.rn

DR. Hosameldin Abdelhady\r\nHasan Mohammed\r\nConsultant and lecture of\r\nradiodaignosis , faculty of\r\nmedicine , assiut university\r\nhospital

Trichobezoar, a ball of hair in the stomach, is an under-diagnosed problem that should be considered in patients presenting with an upper abdominal pain and mass, weight loss, and bowel obstruction especially in young girls with an underlying psychiatric disorder or mental retardation. A trichobezoar extending into the intestine is designated \"Rapunzel syndrome\". Patients with sickle cell disease are susceptible to experiencing pica including, in rare occasions, trichophagia that could lead to formation of a trichobezoar, resulting in serious abdominal complications which are likely to be confused with ordinary vaso-occlusive pain crises. Here we present the rare case of gastric trichobezoar in a 9 year old girl with sickle cell anemia who was initially taken for a painful crisis.

Graduated in Faculty of Medical Sciences at the State University of Campinas (1989). Has Medical Residency in Internal Medicine, Department of Internal Medicine UNICAMP Medical 1991.Residência in Hematology FCM / UNICAMP (1993). Residence in Bone Marrow Transplantation of HEMOCENTRO the Hematology Division (1994) Master\'s degree in Clinical Medicine at the Faculty of Medical Sciences, State University of Campinas (2001) and PhD in Child and Adolescent at the Faculty of Medical Sciences, State University Campinas (2015). Medical responsible for the care of emergency care of blood donors of autologous bone marrow transplant unit and voluntary donors, the apheresis sector transfusion Centro Infantil Boldrini agency since 2007.

Introduction: In the mobilization for autologous transplantation the classical dose of G-CSF is 10 μg/kg of patient body weight in a single dose. There is a theory that higher doses of G-CSF applied twice daily could increase the CD34+ cells collected with a lower number of leukapheresis procedures. rnObjective: The aim of this study was to compare a fractionated dose of 15μg G-CSF /kg and the conventional dose, aimed at reducing the number of leukapheresis procedures required to allow the minimum target of 3 x 106 CD34+ cells /kg to be reached. rnMethods: Patients were divided into two groups. Group 10: patients who received a single dose daily of 10μg G-CSF /kg; and Group 15: patients who received a dose of 15μg G-CSF/kg twice daily. The autologous transplantation was carried out when the minimum number of 3x106 CD34+ cells/kg was reached.rnResults: Group 10 comprised 39 patients and Group 15 26 patients. A total of 146 apheresis procedures were performed: 110 (75.3%) for Group 10 and 36 (24.7%) for Group 15. For Group 10 a median of 3 (1-7) leukapheresis procedures and a mean of 8.89 x106 CD34+ cells/kg bw (± 9.59) were collected whereas for Group 15 the corresponding values were 1 (1-3) and 5.29 x106 (± 4.95). An important statistical difference was in relation to the number of apheresis procedures (p<0.0001).rnConclusions: To collect the minimum target of 3x106 CD34+ cells/kg bw the application of a fractionated dose of 15μg G-CSF /kg decreased significantly the number of leukapheresis procedures performed.rn

Medical specialist in internal medicine from the Universidad Veracruzana, graduated from high specialty Regional Hospital of Veracruz.rnInternist at Mexican Social Security Institute, General Hospital of Zone 71.rnInternist assigned to the intensive care unit, Hospital D\'Maria.rnExpert columnist of the Iberoamerican Society of Scientific Information, with the theme emerging viruses.rnAuthor of several medical articles related to internal medicine and intensive care

Background: Disseminated intravascular coagulation is an entity characterized by activation of the coagulation cascade and endogenous fibrinolysis, which can cause death. Our objectives were to identify the incidence of disseminated intravascular coagulation, its etiologic agents and the correlation between the Apache II score and the one proposed by the International Society on Thrombosis and Haemostasis for the diagnosis of this entity.rnMethods: Retrospective, descriptive, observational study of patients treated in an intensive care unit over a 17-month period. Etiology, age, sex, platelet count, coagulation tests, serum fibrinogen levels and D-dimer quantification were analyzed. The score on the scale proposed by the International Society on Thrombosis and Haemostasis and the Apache II score were calculated.rnResults: 11 patients (7.18 % of the total number treated subjects at the intensive care unit) had a diagnosis of disseminated intravascular coagulation; six were females. Sepsis was the main etiologic agent (four cases). The most affected age group was the 51-60 years group (four cases). The prognosis was bad in seven subjects. Patients with five points or more in the DIC system, but with a low Apache II score had a good prognosis.rnConclusions: The combination of the DIC and the Apache II scores serves for predicting the outcome of patients with severe organ injuries.rn

Dr Edgard LAFIA has completed the degree of Medical Doctor at the Faculty of Health Sciencesrnin Cotonou, Benin. He also graduated in Microbiology at Cheick Anta Diop University of Dakarrnin Senegal, and in Blood Transfusion at St John of God Blood Transfusion Center in Rome, Italyrnand at the University Clinic of St Luc in Brussels. He worked as civil servant for 30 years and isrncurrently external collaborator for the National Agency for Blood Transfusion in Cotonou. Hernhas published 18 papers and is member of three international scientific societies.

With the assistance of the Ministry of Health and the Health Sciences Faculty team onrnAnesthesiology, National Blood Transfusion Agency and Blood Diseases Service at the NationalrnUniversity Hospital of Cotonou organized a training for medical and paramedical personnel fromrn15 to 17 December 2014. This initiative received support from the World Health Organizationrn(WHO) and the United Nations Population Fund (UNFPA). Forty six hospital doctors, dividedrninto 26 from public hospitals and 10 from private hospitals took part in this first training inrnHematology and Blood Transfusion. A French delegation made by Professors PhilippernCOLOMBAT and Jorge DOMENECH from the Faculty of Medicine of Tours, Jean-Yves PYrnand Bernadette MARTENOT from French Blood Establishment gave support to ten teachersrnfrom Health Sciences Faculty in Cotonou for this training. Several themes with clinical casesrnwere the menu of exchanges. Discussions focused in particular the needs of labile blood productsrnin Benin, different types of anemia and their mechanisms and diagnosis, classification ofrnmalignant blood diseases, strategies for prevention or anticipation of labile blood product’srnneeds, and their production and conservation. The most important decision from participants atrnthe end of the training was the establishment of Hematology-Transfusion network. Currently, 24rnpractitioners are listed in the Facebook directory network, of which 11 did not take part in thernformation in December 2014. The effects of the shortage of staff specialized in health servicesrncan therefore be lessened with the support of cooperation between North and South.

O. A. SHAIKHOMAR has completed his PhD at the age of 44 years from Nottingham University. School of Medicine, UK and postdoctoral studies from Umm Al-Qura University, Faculty of Medicine, KSA. He is Associate professor in Human Physiology, Department of Physiology. He is now in the position of Vice Dean for Academic Affair & Development and Chairman of Academic Accreditation Committee . He has published more than 16 papers in reputed journals and has been serving as an editorial board member of repute.rnosamaso98@hotmail.comrn

Recurrent miscarriage is defined as 3 or more consecutive miscarriages, its effects can be distressing and devastating. The condition affects 1% of all women. Sixty percent of recurrent spontaneous abortions are unexplained, and 7% to 25% of unexplained recurrent spontaneous abortions have Antiphospholipid syndrome (APS) as the main risk factor. There are well documented associations between Antiphospholipid antibody and abnormalities of specific cellular components of the blood, such as thrombocytopenia, haemolytic anaemia and or both (Evans Syndrome). rnTo fulfill the objectives of this study a number of 100 Saudi women aged from (15-49 years old) with the history of the presence of three or more consecutive recurrent miscarriage at any gestational age of pregnancy without previous living babies as the case group. A number of 100 Saudi women with non- complicated pregnancy, aged from (15-49 years old) with the presence of at least one or more successful pregnancy, with no history of recurrent miscarriage or any abnormal pregnancy outcome, such as intra-uterine fetal loss), as the control group.rnBlood samples (10 ml) will be collect and separated into serum and plasma for immunological, haematological and biochemical analysis. Data including medical history, gynecologic, clinical history of thrombosis, bleeding and anthropometric information will be collected using a questionnaire. rnThe parameters of thrombocytopenia, autoimmune haemolytic anaemia and Antiphospholipid syndrome (APS) both clinical and analytical (biochemical and haematological) among the subgroups of the study will analyzed. Then, results will be compared with other relevant studies so that conclusions and recommendation formulated.rn